Live-cell imaging system with interchangeable objectives
Automated multiposition time-lapse imaging in brightfield and two fluorescence channels, with interchangeable 2×, 4× and 10× objectives.
Technical summary with specifications and configuration choices.
Features
- Change between 2×, 4× and 10× objectives to balance field of view and detail.
- Acquire brightfield and green/red fluorescence while the culture remains inside the incubator.
- A moving camera and motorised XYZ acquisition keep the sample vessel stationary.
- Software supports confluence, fluorescence cell counting, z-projection, stitching and spheroid/organoid analysis.
Images and workflow examples
Select an image to view it at full size.
Technical specifications
| Imaging modes | Brightfield and two fluorescence channels |
|---|---|
| Objectives | 2×, 4× and 10×; user-interchangeable |
| Fluorescence filters | Green Ex 470/40 nm, Em 540/50 nm; red Ex 562/40 nm, Em 641/75 nm |
| Camera | 5 MP CMOS |
| Field of view at 2× | 2.02 × 1.49 mm |
| Field of view at 4× | 1.41 × 1.05 mm |
| Field of view at 10× | 0.70 × 0.52 mm |
| Acquisition | Motorised XYZ; multiple positions; autofocus and manual focus |
| Culture vessels | Plates up to 96 wells, flasks, dishes and slides; holder-dependent |
| Exports | TIFF, AVI, CSV; JPG, PNG and BMP image export |
| Computer | External PC, Windows 10 or later |
| Recommended incubator | Above 200 L; check internal clearance |
| Dimensions (H × W × L) | 250 × 338 × 412 mm |
| Weight | 9.6 kg |
| Operating environment | 10–40 °C; 20–95% relative humidity |
| Power input | 100–240 V AC, 50/60 Hz |
Applications
- Monitor cell growth and confluence during culture.
- Compare treated and control cultures in time-lapse experiments.
- Follow wound closure, fluorescence changes and cell-cycle progression.
- Observe spheroid cytotoxicity and immune-cell interactions using appropriate assays.
Configurations and accessories
- Specify a single objective or the 2×/4×/10× objective set.
- Select the holder for your plate, flask, dish or microscope slide.
- Include the incubator model, internal dimensions and cable access in your enquiry.
Selection notes
- For research use. Match the objective, vessel holder and fluorescence configuration to your assay when requesting a quotation.
Selection help and applications
Imaging comparison
Choosing a live-cell imaging system →
Compare six imaging systems by vessel workflow, objectives, fluorescence and incubator fit, then specify the configuration for your experiment.
Application example
Tracking wound closure with time-lapse imaging →
A documented NIH-3T3 scratch-assay example: brightfield imaging every 30 minutes for 72 hours, wound-area analysis and interpretation limits.
Application example
Following a cytotoxic response over time →
A documented HeLa-cell example combining brightfield confluence and green fluorescence during a 48-hour nocodazole exposure.
Discuss your experiment
Tell us what you need to measure. Next, add your contact details and review the enquiry before sending.