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Multilayer culture monitoring system

Use brightfield time-lapse imaging to monitor the bottom layer of a culture vessel with up to ten layers.

Technical summary with specifications and configuration choices.

Multilayer culture monitoring system

Features

  • Observe cells at the bottom layer while a multilayer vessel remains in the incubator.
  • Schedule multiple imaging positions and assemble time-lapse videos.
  • Use confluence and growth-curve analysis to support harvest timing.

Select an image to view it at full size.

A multilayer culture vessel on the imaging base inside an incubator.
A multilayer culture vessel on the imaging base inside an incubator.
Illustration of changing confluence during culture; the highlighted area shows image segmentation.
Illustration of changing confluence during culture; the highlighted area shows image segmentation.
Examples of multilayer vessels; the required holder depends on the vessel format.
Examples of multilayer vessels; the required holder depends on the vessel format.

Technical specifications

Multilayer culture monitoring system — Technical specifications
VesselsMultilayer chambers up to ten layers; images acquired from bottom layer
Imaging modeBrightfield
Objective2×
Camera5 MP CMOS, 2592 × 1944 pixels
StageMotorised XYZ
FocusAutofocus and manual
Imaging positionsMultiple
AnalysisTime-lapse, movie creation, confluence and growth curves
ExportsTIFF, AVI
Dimensions (H × W × D)132 × 330 × 450 mm
Weight11.6 kg
Operating environment10–40 °C; 20–95% relative humidity

Applications

  • Track growth during expansion in multilayer culture vessels.
  • Inspect culture development without repeatedly moving vessels outside the incubator.

Configurations and accessories

  • Specify the exact multilayer vessel and its dimensions for holder selection.
  • Alternative holders for multilayer dishes and flasks can be assessed on request.

Selection notes

  • For research use. Match the objective, vessel holder and fluorescence configuration to your assay when requesting a quotation.

Selection help and applications

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