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Low-temperature experiments

Cryo piezo stages for low-temperature microscopy

Coarse positioning, fine scans and optical stability.

Compare stages for reaching a sample, scanning a cold field and keeping an optical target aligned during acquisition.

8 min read · drift and signal calculator

Conceptual cryogenic microscope with coarse positioning, fine scanning and a stationary optical target
Coarse positioning and a local scanner. Conceptual layout.

Begin with the measurement

Choose the travel you need at temperature

Use coarse travel to find a region, then a local scanner for imaging and alignment. Leave fine travel available for drift correction.

A published confocal microscope combines a piezotube scanner with three-axis coarse positioning. It is a useful architecture example. [1]

Our cryogenic range includes both motion scales. See environment selection and cryostat integration for mounting, thermal links and wiring.

Coarse positioning and fine scanning

The three parts of a cryogenic microscopy positioning cycle
TaskWhat you specifyRelevant hardware
Find the regionCoarse XYZ reach, loaded motion and room for approach.Linear positioners and the coarse controller.
Scan the targetFine travel at temperature, calibration, sampling and timing.Open-loop or capacitive-feedback flexure scanner.
Hold for acquisitionRelative motion over the measurement time and bandwidth.The loaded stack, optical support, sensor and feedback configuration.

Linear25-x lists 6 mm travel, approximately 150 nm sensor resolution and 1–2 µm repeatability. Use the optical image and fine scan to locate the target after returning to its region.

Centre the local field within the scanner range. If correction travel runs out, reposition coarsely and register the image again.

How much travel is available cold?

Use the scanner’s travel at the operating temperature and drive voltage. A general ceramic contraction factor cannot establish the range of a complete stage. [3]

Scanner25-xy

55 × 55 µm at 300 K. Ask us for the range at your operating temperature.

Scanner35-xy.Ultra

100 × 100 µm at 300 K; 60 × 60 µm at 4 K, with capacitive sensing. [5]

Allow margin for focus and drift correction. Below 4 K, confirm travel at the actual operating point.

Specify temperature, vacuum grade and magnetic field. Include holders, upper stages, adapters and cable forces in the load.

What does the feedback measure?

The stage sensor measures against its mechanical reference. Motion of an objective support or thermal joint outside that loop can still move the sample relative to the focus.

Liu and colleagues place the objective inside their cryostat to share sample/objective vibration. The design illustrates why relative optical motion matters. [2]

Open-loop local scanning

Suitable for a calibrated local scan with optical re-registration. Check reversal, creep and repeatability under the acquisition sequence.

Capacitive closed-loop scanning

Adds a stage displacement reference. Tune with the real load and include sensor noise and loop dynamics in the budget. [4]

Measure sample-to-focus motion over the acquisition time and bandwidth. Keep RMS jitter separate from peak-to-peak motion and slow drift.

Interactive / Optical alignment budget

Estimate drift and signal loss

Estimate signal loss for a Gaussian point target. Use measured motion where available; the presets are assumptions, not stage specifications.

Relative optical motion assumptions

End-of-hold relative signal

87.9%

Normalized to an aligned, jitter-free target. This is the local jitter average at the final drift offset, not the signal averaged over the full acquisition.

Final relative offset
100.0 nm
Initial signal with jitter
98.0%
Offset at signal threshold
136.8 nm
Modelled time to threshold
27.4 min

The model meets your threshold for this hold. Check measured target motion before choosing a recheck interval.

Discuss this positioning brief
Illustrative optical response versus lateral offsetThe ideal response is centred at zero. Relative drift displaces the response and independent lateral jitter broadens and lowers it. The marker shows the response at the original target position.0%50%100%−750 nm0+750 nm
┄ Ideal, aligned response━ Shifted and jitter-averaged response● Signal at the original target position
Calculation and assumptions

Model: I(r)/I₀ = exp(−ar²), a = 4 ln(2)/FWHM². Independent Gaussian X/Y jitter with standard deviation σ gives a normalized response exp[−ad²/(1+2aσ²)]/(1+2aσ²). Drift offset d = drift magnitude × duration. Assumes an initially centred, unsaturated point target, a stationary Gaussian jitter distribution and a fixed optical response. It omits axial motion, emitter brightness changes, temperature effects and feedback dynamics. Stage resolution and encoder noise are not substituted for measured relative motion.

A practical measurement sequence

  1. 01

    Find after cooldown.

    Find the region optically after cooldown; recheck focus and orientation.

  2. 02

    Let the loaded system settle.

    Track temperature and optical position after coarse motion. Measure the required settling delay.

  3. 03

    Scan and centre locally.

    Scan within the confirmed cold stroke, centre the target and leave correction margin.

  4. 04

    Acquire.

    Finish coarse stepping, then acquire with the drive and sensor state characterised for the experiment.

  5. 05

    Recheck the reference.

    Recheck an optical reference at a cadence set by measured drift. Separate movement from brightness changes.

Troubleshooting motion

Measurements that help distinguish drift, scan errors and relative vibration
Observed behaviourUseful comparisonWhat it helps distinguish
A target slowly leaves the fieldOptical position versus time, temperature and recent motion history.Slow relative drift, thermal recovery and scan-range exhaustion.
Forward and reverse maps differRepeat the same region with reversed scan direction and matched timing.Calibration, hysteresis, creep, dynamics or a changing sample.
The encoder is quiet but the image movesMeasure a reference fixed to the sample in the optical image.Motion outside the stage sensor loop.
A repeatable periodic pattern appearsCompare optical motion spectra with the cryocooler and drive timing.Periodic coupling paths; frequency alone does not prove their origin.

Compare cryogenic stages

Start with coarse travel, local scan field and feedback. We can check adapters, axis order and optical clearance for your cryostat.

Linear25-x cryogenic piezo stage supplied by Precisometer

Coarse positioning

Linear25-x

6 mm coarse X travel for reaching regions after cooldown. Locate the target with the optical signal and fine scanner.

Travel range
6 mm
Max. Load
500 g
Sensor resolution
~150 nm
Published repeatability
1-2 um
Scanner25-xy cryogenic piezo stage supplied by Precisometer

Open-loop XY scanning

Scanner25-xy

Compact open-loop XY scanning. The 55 × 55 µm range is at 300 K; confirm the field at your temperature.

Travel range @300 K
55 x 55 um
Max. Load
200 g
Drive Voltage
Max. 75 V @300 K, Max. 180 V @4 K
Scanner35-xy.Ultra cryogenic piezo stage supplied by Precisometer

XY scanning with feedback

Scanner35-xy.Ultra

Capacitive-feedback XY scanning with 60 × 60 µm travel at 4 K. Pair with the Ultra controller and required environment grade.

Travel range
@300 K: 100 x 100 um; @4 K: 60 x 60 um
Max. Load
500 g
Sensor
Capacitive displacement sensor
Resolution
0.5 nm

Confirm cold travel, repeatability conditions and the exact temperature/vacuum suffix in the quotation.

Include the matching drive and sensor chain

  • MC-NewtonLT.06 — the coarse multi-axis controller option for the cryogenic positioner chain.
  • MC-ArchimedesLT.03 — the controller family for the open-loop cryogenic scanners.
  • MC-ArchimedesLT.03.Ultra — the capacitive-feedback controller for the Ultra scanner chain.
  • FTC25.L** or FTC35.L**, with the appropriate mounting adapters — specify the thermal and mechanical connections for the selected stage family.

Before stacking stages, check interfaces and the load on each lower axis, including everything mounted above it.

From optical target to cold positioning

Choose stages for your cryostat

Send the cryostat envelope, temperature, cold scan field, payload and acquisition time. Include optical geometry and any measured drift or vibration.

Request a cryogenic positioning configuration

Questions before choosing a stage

Can I use room-temperature travel for a 4 K map?

Use the cold specification. Scanner35-xy.Ultra lists 100 × 100 µm at 300 K and 60 × 60 µm at 4 K. Confirm travel again below 4 K.

Does 0.5 nm resolution guarantee a stable optical target?

No. Measure relative sample-to-focus motion over the acquisition time and bandwidth, including motion outside the sensor loop.

Do all cryogenic stages work at 30 mK and in UHV?

Choose the exact grade: standard cryogenic, ULT, UHV or combined UHV.ULT. Confirm the complete sensor and wiring configuration.

Should I choose open-loop or closed-loop scanning?

Open loop can suit calibrated local scans. Capacitive feedback adds a position reference. Compare cold travel, noise, bandwidth and return requirements.

References and datasheets
  1. [1]
    Development of a confocal scanning microscope for fluorescence imaging and spectroscopy at variable temperatures

    Hu, Moran and Woehl (2019), Review of Scientific Instruments 90, 043702. A published microscope architecture combining coarse low-temperature positioning with a local piezo scanner.

  2. [2]
    Cryogenic photoluminescence imaging system for nanoscale positioning of single quantum emitters

    Liu et al. (2017), Review of Scientific Instruments 88, 023116. Objective/sample geometry and common-mode vibration in cryogenic optical measurements.

  3. [3]
    Temperature-dependent behavior of piezo actuators

    PI Ceramic, technology reference. Temperature-dependent displacement; it does not establish a universal cold-range multiplier for a finished stage.

  4. [4]
    Piezo controllers and piezo drivers

    Physik Instrumente, technology reference. Position feedback, controller tuning and dependence on the payload and mechanical system.

  5. [5]
    Cryogenic Motion — Scanner.Ultra

    MultiFields, manufacturer product information; see linked Precisometer model sheets for the quoted configuration. Scanner architecture, capacitive sensing, temperature-dependent travel and environment variants.

Product cards link the model sheets. Research examples and the optical model illustrate design choices; their performance is not a stage specification.