Excitation–emission matrix spectroscopy
SmartFluo-Pro 3D Fluorescence Spectrometer
SmartFluo-Pro records excitation–emission matrices (EEMs) to characterise fluorescent dissolved organic matter in water. A 150 W continuous xenon source and 200–800 nm excitation and emission ranges support routine water-quality research and treatment-process comparisons.
- Excitation & emission
- 200–800 nm
- Spectral bandwidth
- 5 nm
- Integration time
- 100 µs–24 s
- Source
- 150 W xenon
Excitation × emission × intensity
Read the fluorescence fingerprint
An excitation–emission matrix records fluorescence intensity across both excitation and emission wavelength. The resulting surface or contour map helps compare the fluorescent components of dissolved organic matter, including protein-like and humic-like signals.
SmartFluo-Pro supports excitation, emission, synchronous, stability and three-dimensional fluorescence scans. Saved measurement methods and extraction of spectra from the EEM support repeated sample comparisons and review of individual spectral features.
Measurement & software
- Excitation, emission, synchronous and 3D EEM scan modes
- Stability scans and three-dimensional synchronous fluorescence
- Contour views with excitation, emission and synchronous spectra extracted from 3D data
- Saved measurement methods and software data processing
Applications
- Natural-water and dissolved organic matter characterisation
- Municipal wastewater and treatment-process comparisons
- Industrial wastewater fluorescence screening
- Studies of organic-matter transformation and biodegradation
SmartFluo-Pro Specifications
| Light source | 150 W continuous xenon lamp |
|---|---|
| Excitation range | 200–800 nm |
| Emission range | 200–800 nm |
| Excitation bandwidth | 5 nm |
| Emission bandwidth | 5 nm |
| Excitation monochromator | 200 mm focal length; Czerny–Turner design |
| Grating | 1800 lines/mm; 400 nm blaze |
| Wavelength accuracy | ±1 nm |
| Wavelength repeatability | ±0.5 nm |
| Integration time | 100 µs–24 s |
|---|---|
| Specified detection limit | < 0.1 µg/L for quinine sulfate in 0.1 mol/L sulfuric acid |
| Sample holder | Standard quartz cuvette |
| Scan modes | Excitation, emission, stability, synchronous, 3D fluorescence and 3D synchronous fluorescence |
| Dimensions | 620 × 415 × 300 mm (L × H × W) |
| Weight | 25 kg |
The detection limit is specified for a quinine-sulfate standard under the stated conditions. It is not a universal detection limit for all dissolved organic compounds; sample matrix, measurement settings and processing affect the result.
SmartFluo-Pro datasheet (PDF)Planning a research fluorescence experiment?
Explore excitation, detector assemblies, sample holders and liquid-nitrogen cryostats listed for OmniFluo900 research platforms. Select compatibility around the instrument and measurement.
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