Dual-channel 3D imaging module
SPINDLE²™
Capture two independently adaptable channels simultaneously and turn an existing microscope into a multicolor, multimodal 3D imaging system.
Double Helix Optics · microscope retrofit
2 channels
Simultaneously imaged
1 camera
Combined acquisition path
Single shot
Instantaneous 3D capture
Any DHO mask
Interchangeable phase masks
Why SPINDLE²™
Add depth without rebuilding your microscope.
DHO engineered point-spread functions embed axial information directly into the spatial shape of each emitter. This captures 3D information in a single exposure and can reduce or eliminate repetitive axial scanning.
Explore DHO technologyTwo simultaneously imaged, individually adaptable optical channels
Mix imaging modalities, colors, phase masks, or depth ranges between channels
Single-camera architecture for simultaneous multichannel capture
Bypass mode for single-channel experiments and conventional 2D imaging
Compact form factor compatible with most scientific microscopes and cameras
Tight integration with 3DTRAX® processing and analysis software
Imaging workflow
From photons to quantitative 3D data.
Connect
Add the optical module to the microscope emission path and connect the selected scientific camera.
Encode depth
An interchangeable phase mask engineers the point-spread function so axial position is encoded in each image.
Capture
Acquire instantaneous 3D information without mechanically scanning through every axial plane.
Reconstruct
3DTRAX® localizes, tracks, reconstructs, visualizes, and exports quantitative 3D results.
Best-fit experiments
Built for spatial questions.
Final configuration depends on emission bands, camera format, microscope interface, phase mask, and the required axial range.
Simultaneous multicolor
Observe two labels at the same instant without sequential acquisition.
Multimodal imaging
Configure each channel for a different PSF, polarization, or imaging mode.
FRET and smFRET
Preserve timing relationships while acquiring donor and acceptor channels.
Spatial biology
Capture multiplexed targets through an extended 3D volume.
Compatible applications
Use the sample preparation and illumination method appropriate to your experiment, then select the phase mask and reconstruction workflow for the measurement.
Compare the platform
SPINDLE®
Choose the single-channel module when one optical path is sufficient.
View SPINDLE®Need help specifying a system?
Share your microscope, camera, fluorophores, imaging depth, and application. We will help identify a suitable module and phase-mask configuration.
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