Single-channel 3D imaging module
SPINDLE®
A compact, cost-effective route from conventional 2D microscopy to single-shot 3D imaging with the full library of DHO phase masks.
Double Helix Optics · microscope retrofit
1 channel
Focused, economical path
Single shot
Instantaneous 3D capture
Any DHO mask
Interchangeable phase masks
Bypass mode
Conventional 2D imaging
Why SPINDLE®
Add depth without rebuilding your microscope.
DHO engineered point-spread functions embed axial information directly into the spatial shape of each emitter. This captures 3D information in a single exposure and can reduce or eliminate repetitive axial scanning.
Explore DHO technologySingle-channel optical path for robust, repeatable 3D imaging
Compatible with the full range of interchangeable DHO phase masks
Compact and cost-effective retrofit for an existing microscope
Bypass mode for conventional 2D image acquisition
Compatible with most scientific optical microscopes and cameras
Tight integration with 3DTRAX® processing and analysis software
Imaging workflow
From photons to quantitative 3D data.
Connect
Add the optical module to the microscope emission path and connect the selected scientific camera.
Encode depth
An interchangeable phase mask engineers the point-spread function so axial position is encoded in each image.
Capture
Acquire instantaneous 3D information without mechanically scanning through every axial plane.
Reconstruct
3DTRAX® localizes, tracks, reconstructs, visualizes, and exports quantitative 3D results.
Best-fit experiments
Built for spatial questions.
Final configuration depends on emission bands, camera format, microscope interface, phase mask, and the required axial range.
3D SMLM
Reconstruct nanoscale structures from engineered-PSF localization data.
Particle tracking
Follow fast-moving targets through three dimensions over time.
Deep-focus imaging
Reduce axial scanning when imaging extended structures.
Routine 3D workflows
Standardize a single optical path for a focused application.
Compatible applications
Use the sample preparation and illumination method appropriate to your experiment, then select the phase mask and reconstruction workflow for the measurement.
Compare the platform
SPINDLE²™
Choose the dual-channel module for simultaneous multicolor or multimodal work.
View SPINDLE²™Need help specifying a system?
Share your microscope, camera, fluorophores, imaging depth, and application. We will help identify a suitable module and phase-mask configuration.
Discuss your experiment Manufacturer product page